Journal: Chinese Medical Journal

Article Title: UBE2C affects breast cancer proliferation through the AKT/mTOR signaling pathway

doi: 10.1097/CM9.0000000000001708

Figure Lengend Snippet: Suppressing UBE2C expression affects BC cell proliferation and invasion. (A) mRNA and protein expression levels of UBE2C detected after siRNA transfection in MDA-MB-231 and MCF-7 cells; (B) CCK-8 assay was performed to assess cell proliferation in MDA-MB-231 and MCF-7 cells transfected with UBE2C siRNA (siUBE2C-1 and siUBE2C-2) and control siRNA. ∗ P < 0.050; (C) Knockdown of UBE2C significantly decreased the invasion of two cell lines (original magnification × 400). † P < 0.010, ‡ P < 0.001. BC: Breast cancer; UBE2C: Ubiquitin-conjugating enzyme E2C.

Article Snippet: When the tumor cells reached the required confluency, we treated them with small interfering RNA (siRNA) (Ribobio, Guangzhou, China) mixed with Lipofectamine 2000 transfection reagent (Invitrogen, CA, USA).

Techniques: Expressing, Transfection, CCK-8 Assay, Control, Knockdown, Ubiquitin Proteomics

Journal: American Journal of Cancer Research

Article Title: Long noncoding RNA TOB1-AS1, an epigenetically silenced gene, functioned as a novel tumor suppressor by sponging miR-27b in cervical cancer

doi:

Figure Lengend Snippet: TOB1-AS1 inhibited cell growth and invasion. A. PCR assay was used to verify the expression of TOB1-AS1 in cells. B. Overexpression of TOB1-AS1 in HeLa cells suppressed proliferation, while knockdown of TOB1-AS1 promoted proliferation of C33A cells. C. Upregulation of TOB1-AS1 induced cell cycle arrest at G1 phase; Silencing of TOB1-AS1 promoted cell cycle progression from G1 phase to S phase. D. Apoptotic cells were increased by pcTOB1-AS1 and decreased by si-RNA1 and si-RNA2. E. The invasion ability were assayed using the transwell assay in HeLa and C33A cells. pcDNA3.1, negative control; pcTOB1-AS1, TOB1-AS1 expressing plasmid; si-ctrl, siRNA control; si-RNA1/2, siRNAs for knocking down TOB1-AS1. *P<0.05; **P<0.01.

Article Snippet: Plasmid construction and oligonucleotide transfection Small interfering RNA (siRNA) and non-specific control siRNA (si-ctrl) were synthesized by GenePharma Co., Ltd. (Shanghai, China) and transfected into cells by using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA).

Techniques: Expressing, Over Expression, Knockdown, Transwell Assay, Negative Control, Plasmid Preparation, Control

Journal: American Journal of Cancer Research

Article Title: Long noncoding RNA TOB1-AS1, an epigenetically silenced gene, functioned as a novel tumor suppressor by sponging miR-27b in cervical cancer

doi:

Figure Lengend Snippet: TOB1-AS1/miR-27b axis was involved in cell growth in vivo. (A) The protein levels of Cyclin D1, Bax, E-cadherin, and N-cadherin were detected by western blot analysis. (B) Representative xenograft tumors obtained from mouse injected with pcTOB1-AS1 or pcDNA3.1. (C and D) Construction of TOB1-AS1 stably expressing cells led to an decreasing of tumor volume (C) and weight (D). (E and F) The expression of TOB1-AS1 (E) and miR-27b (F) in xenograft tumors were measured by qPCR. (G) A schematic diagram deciphering the mechanism underlying the effect of epigenetically silenced TOB1-AS1 on the expression of miR-27b and cervical cancer progression. mim con, mimic control; inh con, inhibitor control. pcTOB1-AS1, TOB1-AS1 expressing plasmid; si-ctrl, siRNA control; si-RNA1, siRNA for knocking down TOB1-AS1; **P<0.01.

Article Snippet: Plasmid construction and oligonucleotide transfection Small interfering RNA (siRNA) and non-specific control siRNA (si-ctrl) were synthesized by GenePharma Co., Ltd. (Shanghai, China) and transfected into cells by using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA).

Techniques: In Vivo, Western Blot, Injection, Stable Transfection, Expressing, Control, Plasmid Preparation